Mutagenic and Induced Antibiotic Resistance of Acridine Dye on E. Coli and Proteus mirabilis Isolated from Clinical Samples.

Abstract

Acridine orange has been associated with chemical mutagenic compound that can cause an increase in the frequency of mutations. Its mutagenic influence varies with its potency because of its ability to penetrate the cell, its reactivity with DNA and its general toxicity. This study evaluated the extent to which acridine orange influence microbial resistance to antibiotic. Of 20 sensitive isolates of E. coli and P. mirabilis used, 100% of the isolates were resistant to ampicillin-sulbactam, 80% to amoxicillin, 20% to cefalexin and non (0%) were resistant to ciprofloxacin, nitrofurantoin, ofloxacin, chloramphenicol, ceftriazone and gentamicin. A total of 70% of the E. coli and 80% of P. mirabilis isolated were resistant to cefuroxime, 90% of the E. coli and 100% of P. mirabilis were resistant to ceftazidine, 50% of
the E. coli and 80% of P. mirabilis to ampicillin, 80% of the E. coli and 100% of P. mirabilis to amoxicillin-clavulanic acid, 20% of the E. coli and 80% of P. mirabilis to tetracycline, 30% of the E. coli and 50% of P. mirabilis to nalidixic acid respectively after incubation at 37 0 C for 72hrs in acridine dye and overnight broth culture at the ratio of 5:1. This study indicated that acridine orange shows a significant mutagenic antibiotic resistant property to microorganism.