Status of some biochemical markers in Sprague dawley rats following sub-chronic consumption of alum- treated water

Abstract

Alum is a double salt of aluminum and potassium sulfates. The aim of this study was to investigate the status of some biochemical markers in Sprague-dawley rats following sub-chronic consumption of alum- treated water. It consisted of 21 rats of 3–4 months old weighing roughly 122 ± 0.5 g. They were divided into three groups at random with 7 rats / group. The control group received 15 ml of distilled water daily for 30 days, while experimental groups one and two received 15ml of 0.04% concentration of alumtreated water for 15 days daily and 30 days respectively. Following this, 5 ml of  blood specimen was withdrawn via cardiac puncture and introduced into corresponding lithium heparin anti-coagulated bottles. These specimens were spun at 1,500 revolutions per minute for 10 minutes using an 800 D macro centrifuge to extract the plasma that were utilized for the measurement of alanine aminotransferase, aspartate aminotransferase (colorimetric method), urea (urease Berthelot method), creatinine (Jaffe reaction method) and C-reactive protein (Latex turbidimetry method). Using SPSS 23.0 to perform statistical analysis on the study's findings, it was discovered that there were no significant differences between the experimental group one and the control group for all the measured biochemical parameters (p>0.05). However, in comparison to the control group, the results of the experimental group two revealed significant elevations (p<0.05) in revealed significant elevations (p<0.05) in plasma levels of alanine aminotransferase, aspartate aminotransferase and C-reactive protein. It is concluded that the plasma concentrations of alanine aminotransferase, aspartate aminotransferase and C-reactive protein may be altered in Sprague-dawley rats following consumption of 15 ml of 0.04% concentration of alum- treated water for 30 days. Thus, it is recommended that this study in a well guided manner be carried out in humans to ascertain if the findings will be consistent.