Antimicrobial Activities of Sokoto Prepared Black Soap (Sabulun Salo) on Clinical Isolates of Candida albicans, Staphylococcus aureus and Escherichia coli.

Abstract

In general, Sabulun salo can be any antifungal and antibacterial soap with added antimicrobial active ingredients. These chemicals kill bacteria and other microorganisms, although they are not as effective at inactivating viruses as any other soap. The antifungal and antibacterial activity of Sabulun salo was determined on Candida albicans, Staphylococcus aureus and Escherichia coli isolated from gastric and skin infections. This project aimed to determine the activity of sabulun salo on some fungal, gram- negative and gram-positive bacteria. Confirmation of isolates was performed using standard microbiological methods. Different concentrations (12.5mg/ml, 25mg/ml, 50mg/ml and 100mg/ml) of each soap were tested by disc diffusion method against Candida albicans, Staphylococcus aureus and Escherichia coli respectively, and the zone of inhibition was recorded. Sabulun salo was found to be effective against fungal strains and some bacterial strains tested with a peak zone of inhibition of 27mm for Staphylococcus aureus and 20 mm for Candida albicans at the highest dilution used at 100mg/ml, followed by Escherichia coli with 10mm at 100 mg/ml. The minimum inhibitory concentration results show that Sabulun salo has a minimum inhibitory concentration of 12.5mg/ml for Staphylococcus aureus, Escherichia coli and Candida albicans, respectively, and a minimum bactericidal concentration of 25mg /ml, 12.5mg/ml and 25mg/ml corresponds to Candida albicans, Staphylococcus aureus and Escherichia coli. These sabulun salo have fungicidal and bactericidal effects, especially at high concentrations; They need to be used in high concentrations for them to be effective.