Determination of Antimicrobial Activities of Crude Extract (Juice) of Citrus Aurantifolia (LemunTsami) on Some Selected Bacteria of Different Gram Reaction (StaphylococcusaureusandEscherichiacoli).

Abstract

Citrus aurantifolia is a nutrient-rich plant that is beneficial as an antibacterial agent as well as in the treatment of diverse ailments. In this study, the antimicrobial activity of the crude extract (juice) of C. aurantifolia was compared against Ciprofloxacin on two bacterial isolates (S. aureus and E. coli). The activity patterns of the e x t r a c t a n d t h e s t a n d a r d a n t i b i o t i c s (Ciprofloxacin) on the isolates were determined using standard microbiological techniques. Highly significant activity (sensitivity) of the extract (juice) was observed on both isolates; with S. aureus recording a greater susceptibility; while E. coli responded moderately at the same concentrations (1/32, 1/16, 1/8, 1/4, 1/2 serial dilutions and undiluted form). The susceptibility of these organisms of different gram reactions to the extract is proof of the plant's potential to be used as a source of broad-spectrum drug against these bacteria. C. aurantifolia crude extract particularly the juice, therefore, may be employed as potential source of novel antibacterial agent to prevent the present antibiotic resistance perceived threat.